VENOUS BLOOD SAMPLING
Three methods of sampling: Capillary, Venous, Arterial
Aims
To obtain blood sample by using aseptic technique in order to reduce the nosocomial infection.
Responsibility
Doctors and nursing staff.
*Use caution in patients with coagulation disorder.
*Differentiate between arteries and veins, and avoid the site that can be used for cannulation.
Procedure
Procure the required equipments before sampling:
- Gloves
- Spirit solution
- Povidone iodine solution
- Needles gauge no. 21 or 23
- Syringes
- Blood sample containers
- Dry sterile cotton swab
- Tourniquet
- Dextrose 10 % or Sucrose 25% solution
Start with:
- Identify the baby.
- Wash hands. Put on the gloves.
- In case of neonates, administer dextrose 10 % or sucrose 25% by a cotton wick orally for two minutes prior to procedure.
- Identify a suitable vein and gently apply torniquet.
- Wipe the skin with spirit swab and allow the skin to dry.
- Puncture the site by inserting a 21 or 23 gauge needle into the vessel, puncture should be done at 15 to 45 degree angle.
- Collect the blood in the appropriate blood sample containers. For blood culture, collect the blood in sterile syringe and puncture the sterile container with the needle attached to syringe.
- Remove the tourniquet, withdraw the needle, and apply pressure over the puncture site until haemostasis occurs.
- Discard needle in a safe container.
- Wash hands.
- Label the container with the baby's name and registration number.
Look out for complications like hematoma, infection, haemorrhage, needle injury to adjacent structures and pain
References
Core Curriculum for Neonatal Intensive Care Nursing by Verklan and Walden, 3rd edition (2004), Pratical Procedure for Newborn Nursery by Deoari, Paul et.al., 3rd edition (2010), Comprehensive Neonatal Nursing Care by Kenner and Lott, 5 edition (2014).
Document Information
Version: 1.0 (Archived)
Published: August 3, 2015
Contributors: Nurse Ajeeta George, JK Lone Hospital, SMS Medical College, Jaipur
View: Current Version