Venous Blood Sampling
Obtain blood sample by aseptic technique in order to reduce nosocomial infection.
Responsibility
Doctors and nursing staff.
- Use caution in patients with coagulation disorder.
- Differentiate between arteries and veins, and avoid the site that can be used for cannulation.
PROCEDURE
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Required equipments before sampling:
- Gloves
- Spirit solution and povidone iodine solution
- Needles gauge no. 21 or 23 and Syringes
- Blood sample vials
- Dry sterile cotton swab
- Dextrose 10 % or Sucrose 25% solution
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Identify the baby.
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Wash hands. Put on the gloves.
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In case of neonates, administer dextrose 10 % or sucrose 25% by a cotton wick orally for two minutes prior to procedure.
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Identify a suitable vein, wipe the skin with spirit swab and allow the skin to dry.
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Puncture the site by inserting a 21 or 23 gauge needle into the vessel, puncture should be done at 15 to 45 degree angle.
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Collect the blood in the appropriate blood sample containers. For blood culture, collect the blood in sterile syringe and puncture the sterile container with the needle attached to syringe.
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Withdraw the needle, and apply pressure over the puncture site until haemostasis occurs.
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Destroy the needle and discard in blue or white waste container.
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Wash hands and label the blood vial with the baby's name and registration number.
Look out for complications like hematoma, infection, hemorrhage, needle injury to adjacent structures and pain.
References
- Core Curriculum for Neonatal Intensive Care Nursing by Verklan and Walden, 3rd edition (2004)
- Pratical Procedure for Newborn Nursery by Deoari, Paul et.al., 3rd edition (2010)
- Comprehensive Neonatal Nursing Care by Kenner and Lott, 5 edition (2014).
Document Information
Version: 2.0
Published: August 20, 2015
Revised by: Johanne M. E. Huurnink, Dr Chetan Meena
Contributors: Nurse Ajeeta George, JK Lone Hospital, SMS Medical College, Jaipur
Previous Versions: v1.0